skeletal muscle differentiation tool  (ATCC)

Journal: International Journal of Molecular Medicine

Article Title: Recombinant ADAMTS-1 promotes muscle regeneration accompanied by downregulation of Notch signaling

doi: 10.3892/ijmm.2025.5718

Figure Lengend Snippet: Effect of rADAMTS-1 on MuSCs following BaCl 2 -induced TA muscle injury. Groups included NC, BaCl 2 , and two treatment groups receiving rADAMTS-1 (L) and (H). Daily intraperitoneal injections of rADAMTS-1 were initiated immediately after injury and continued until the designated time point for analysis. (A) Flow cytometry analysis of MuSCs isolated from injured TA muscles, defined by surface marker expression (CD45 − /CD31 − /Sca1 − /VCAM1 + ). (B) Representative gating strategy for MuSCs across experimental groups at different time points. (C) Relative number of MuSCs normalized to the NC group over time. Statistical significance was determined using one-way analysis of variance, followed by Tukey's post hoc test: * P<0.05, ** P<0.01 and *** P<0.001 vs. NC; ## P<0.01 and ### P<0.001 vs. BaCl 2 group. (D) Representative immunofluorescence images of Pax7 + cells in injured TA muscle at different time points (scale bar, 25 μ m). (E) Quantification of Pax7 + cell percentages over time. Statistical significance was determined using one-way analysis of variance with Tukey's post hoc test: * P<0.05 and *** P<0.001 vs. NC; ### P<0.001 vs. BaCl 2 ; ††† P<0.001 vs. rADAMTS-1 (L). ADAMTS-1, a disintegrin and metalloproteinase with thrombospondin motifs 1; rADAMTS-1, recombinant ADAMTS-1; MuSCs, skeletal muscle satellite cells; BaCl 2, barium chloride; TA, tibialis anterior; NC, non-injured control; rADAMTS-1 (L), rADAMTS-1 at 5 mg/kg; rADAMTS-1 (H), rADAMTS-1 at 10 mg/kg; CD, cluster of differentiation; Sca1, stem cell antigen 1; VCAM1, vascular cell adhesion molecule; Pax7, paired box protein.

Article Snippet: For differentiation, the cells were treated with a skeletal muscle differentiation tool (cat. no. PCS-950-050; ATCC) upon reaching 90% confluence.

Techniques: Flow Cytometry, Isolation, Muscles, Marker, Expressing, Immunofluorescence, Recombinant, Control

Journal: International Journal of Molecular Medicine

Article Title: Recombinant ADAMTS-1 promotes muscle regeneration accompanied by downregulation of Notch signaling

doi: 10.3892/ijmm.2025.5718

Figure Lengend Snippet: Effects of rADAMTS-1 on proliferation and differentiation of primary skeletal muscle cells. (A) Dose-dependent proliferative effects of rADAMTS-1 (0.001-10 ng/ml) on primary skeletal muscle cells, assessed using the bromodeoxyuridine assay. (B) Dose-dependent effect of rADAMTS-1 (0.001-10 ng/ml) on the differentiation of primary skeletal muscle cells. Quantification of myotube length following differentiation (scale bar, 200 μ m). (C) Western blot analysis showing the expression of myogenic and ADAMTS-1 related markers (MyoD, MyoG, ADAMTS-1, NICD, Hes-1, and β-actin) following rADAMTS-1 treatment at a concentration of 10 ng/ml, indicating its promotion of early-stage myogenic differentiation through inhibition of NICD signaling. Each data point represents an individual measurement; error bars indicate standard deviation. Statistical significance was determined using one-way analysis of variance followed by Tukey's honestly significant difference post hoc test and is indicated as follows: (A) * P<0.05 vs. Con group; (B) ** P<0.01 and *** P<0.001 vs. Con group; (C) * P<0.05 and ** P<0.01 vs. Con group. ADAMTS-1, a disintegrin and metalloproteinase with thrombospondin motifs 1; rADAMTS-1, recombinant ADAMTS-1; MyoD, myoblast determination protein 1; MyoG, myogenin; NICD, Notch intracellular domain; HES-1, hairy and enhancer of split-1; Con, control.

Article Snippet: For differentiation, the cells were treated with a skeletal muscle differentiation tool (cat. no. PCS-950-050; ATCC) upon reaching 90% confluence.

Techniques: BrdU Staining, Western Blot, Expressing, Concentration Assay, Inhibition, Standard Deviation, Recombinant, Control